Restriction Enzymes
Thermo Scientific™ Tru1I (MseI) (10 U/μL)
Cut at T^TAA sites with Tru1I (MseI) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: MseI, Tru9I).
Thermo Scientific™ PagI (BspHI) (10 U/μL)
The PagI (BspHI) restriction enzyme recognizes T^CATGA sites and cuts best at 37°C in O buffer (Isoschizomers: BspHI, CciI, RcaI).
Thermo Scientific™ FastDigest BseMI
Cut at GCAATG(2/0)^ sites with Thermo Scientific™ FastDigest™ BsrDI (BseMI), which performs best with best using universal FastDigest Buffer (Isoschizomers: Bse3DI).
Thermo Scientific™ EcoRI, HC (50 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ BseMI (BsrDI) (5 U/μL)
Cut at GCAATG(2/0)^ sites with BseMI (BsrDI) restriction enzyme, which performs best at 55°C in R buffer (Isoschizomers: Bse3DI, BsrDI).
Thermo Scientific™ FastDigest XmiI
Cut at GT^MKAC sites with Thermo Scientific™ FastDigest™ AccI (XmiI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FblI).
Thermo Scientific™ FastDigest SgsI
Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).
Thermo Scientific™ FastDigest SfaAI
Cut at GCGAT^CGC sites with Thermo Scientific™ FastDigest™ AsiSI (SfaAI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ SsiI (AciI) (10 U/μL)
Cut at CCGC(-3/-1)^ sites with SsiI (AciI) restriction enzyme, which functions best at 37°C in O buffer (Isoschizomers: AciI, BspACI).
Thermo Scientific™ MnlI (10 U/μL)
The MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in G buffer.
Thermo Scientific™ Eco31I (BsaI) (10 U/μL)
The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).
Thermo Scientific™ FastDigest Alw44I
Cut at G^TGCAC sites with Thermo Scientific™ FastDigest™ ApaLI (Alw44I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: VneI).
Thermo Scientific™ NotI (10 U/μL)
The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).
Thermo Scientific™ FastDigest Eco147I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ BsuRI (HaeIII) (10 U/μL)
Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI).